Baculovirus Surface Display Using Infuenza Neuraminidase (NA) Transmembrane Anchor

Surface display of heterologous proteins in Bacillus thuringiensis using a peptidoglycan hydrolase anchor

BACKGROUND Previous studies have revealed that the lysin motif (LysM) domains of bacterial cell wall-degrading enzymes are able to bind to peptidoglycan moieties of the cell wall. This suggests an approach for a cell surface display system in Gram-positive bacteria using a LysM-containing protein as the anchoring motif. In this study, we developed a new surface display system in B. thuringiensi...

Baculovirus Display: A Novel Tool for Vaccination

Baculovirus surface display of Theileria parva p67 antigen preserves the conformation of sporozoite-neutralizing epitopes.

Theileria parva is an intracellular protozoan parasite that causes East Coast fever, a severe lymphoproliferative disease in cattle. Previous attempts to produce recombinant sporozoite surface antigen (p67) in bacterial or insect cells for vaccine purposes have not resulted in a correctly folded protein. Here, we report the expression of N- and C-terminal domains of p67 fused to the baculovirus...

Use of hr 3 enhancer and P 74 TM domain in baculovirus surface display

1 The Key Laboratory of Cell Proliferation and Differentiation of Ministry of Education and The State Key Laboratory of Biomembrane and Membrane Bio-engineering, College of Life Sciences, Peking University, Beijing 100871, P. R. China, The Center for Theoretical Biology, Peking University, Beijing 100871, P. R. China, Institute of Virology, Zhejiang Provincial Center For Disease Prevention and ...

Baculovirus surface display: construction and screening of a eukaryotic epitope library.

The baculovirus expression system was utilized to serve as a tool for ligand selection, demonstrating the applicability of the system to the generation and screening of eukaryotic expression libraries. The HIV-1-gp41 epitope 'ELDKWA', specific for the neutralizing human mAb 2F5, was inserted into the antigenic site B of influenza virus hemagglutinin and expressed on the surface of baculovirus i...